Purified sperm is sex sorted at Sexing Technologies into X-bearing chromosome (female) and Y-bearing chromosome (male) populations. These purified subpopulations are frozen for future use in artificial breeding.
Sperm is sorted by identifying differences between the X- and Y- bearing sperm. The X- chromosome (female) contains about 3.8% more DNA than the Y- chromosome in cattle. This DNA difference in DNA content can be used to sort the X- from the Y- breaing sperm.